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7 aad  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences 7 aad
    7 Aad, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aad/7-AAD/pmc13011060-429-10-12
    Average 96 stars, based on 1 article reviews
    7 aad - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    FACS:

    Article Title: Cryptococcus neoformans responds to presence of mycobacterium by diversifying its morphologies and remodelling its capsular material
    Article Snippet: Cells were subsequently stained with anti-CD11b (BUV395, BD Biosciences), anti-CD11c (BV711, BD Biosciences), anti-Siglec-F (CD170 Super Bright 436, Fisher Scientific), anti-CD45 (PerCP-Cy 5.5, BD Biosciences) and anti-F4/80 (565410, BD Biosciences) at 4°C for one hour. .. Cells were then washed with FACS buffer containing 0.5% bovine serum albumin, 5mM EDTA with 7-AAD, before being measured by Cytek Aurora Flow Cytometer (Cytek Biosciences, Amsterdam, The Netherlands) and analysed on FlowJo (version 10.9.0 for macOS). ..

    Article Title: Human LY9 governs CD4 + T cell IFN-γ immunity to Mycobacterium tuberculosis .
    Article Snippet: Masato Ogishi*, Julia Puchan†, Rui Yang†, Andrés Augusto Arias†, Ji Eun Han†, Tina Nguyen†, Rebeca GutiérrezCózar†, Clément Conil†, Yoann Seeleuthner, Darawan Rinchai, Peng Zhang, Khoren Ponsin, Matthieu Chaldebas, Yi Feng, AnnaLena Neehus, Ottavia M. Delmonte, Taushif Khan, Nils Landegren, Daniel Eriksson, Jonathan Bohlen, Jessica N. Peel, Iris Fagniez, Simon J. Pelham, WeiTe Lei, Maya Chrabieh, Candice Laine, Hind Ouair, Ibtihal Benhsaien, Ahmed Abid, Ismail Abderrhamani Ghorfi, Hicham Souhi, Hanane Ouazzani, Rafik Aniss, D. Sean Riminton, Olle Kämpe, Stuart E. Turvey, Nico Marr, Luigi D. Notarangelo, Nevin Hatipoglu‡, Aziz Bousfiha‡, Tayfun Ozcelik‡, Jamila El Baghdadi‡, Aurelie Cobat§, Cindy S. Ma§, Laurent Abel§, Anne Puel§, Jacinta Bustamante§, Pablo Engel§, Philippe Gros§, Stuart G. Tangye¶, Federica Sallusto¶, Stéphanie BoissonDupuis*#, JeanLaurent Casanova*#

    Article Title: Somatic Miwi2 Modulates Mitochondrial Function in Airway Multiciliated Cells and Exacerbates Influenza Pathogenesis
    Article Snippet: .. After incubation, cells were washedwith PBS, centrifuged at 300xg, and resuspended in FACS buffer supplemented with 7-AAD and analyzed on the Cytek Aurora. e3 iScience 28, 112291, April 18, 2025 qRT-PCR RNA isolation frommouse whole lung homogenate was performed using the Qiagen RNeasymini kit according to themanufacturer’s instructions. qRT-PCR was performed using the TaqMan and QuantStudio 3 (Applied Biosystems 4392938) according to the manufacturer’s instructions. ..

    Flow Cytometry:

    Article Title: Cryptococcus neoformans responds to presence of mycobacterium by diversifying its morphologies and remodelling its capsular material
    Article Snippet: Cells were subsequently stained with anti-CD11b (BUV395, BD Biosciences), anti-CD11c (BV711, BD Biosciences), anti-Siglec-F (CD170 Super Bright 436, Fisher Scientific), anti-CD45 (PerCP-Cy 5.5, BD Biosciences) and anti-F4/80 (565410, BD Biosciences) at 4°C for one hour. .. Cells were then washed with FACS buffer containing 0.5% bovine serum albumin, 5mM EDTA with 7-AAD, before being measured by Cytek Aurora Flow Cytometer (Cytek Biosciences, Amsterdam, The Netherlands) and analysed on FlowJo (version 10.9.0 for macOS). ..

    Article Title: Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration
    Article Snippet: Briefly, cells were harvested by trypsinization, washed once with annexin V binding buffer (BD Biosciences, USA), and incubated with Annexin V-FITC (BD Biosciences, USA) for 20 min at 4 oC. .. After one wash, cells were labeled with 7-AAD for 15 min at 4oC and immediately analyzed using a Cytek Aurora spectral flow cytometer, with SpectroFlo ® software (Cytek Bioscience, USA). .. In parallel, viability was also assessed using the Cell Death Detection ELISA PLUS kit (Roche, Switzerland), which quantifies cytoplasmic histone-associated DNA fragments as a hallmark of apoptosis.

    Article Title: Cryptococcus neoformans responds to presence of Mycobacterium by diversifying its morphologies and remodelling its capsular material
    Article Snippet: Then, cells were blocked by Fc-blocking buffer (anti-CD16/32, BD Biosciences, Wokingham, UK) for 10 min at 4 °C. .. Cells were subsequently stained with anti-CD11b (BUV395, BD Biosciences), anti-CD11c (BV711, BD Biosciences), anti-Siglec-F (CD170 Super Bright 436, Fisher Scientific), anti-CD45 (PerCP-Cy 5.5, BD Biosciences) and anti-F4/80 (565410, BD Biosciences) at 4 °C for 1 h. Cells were then washed with flow cytometry cell buffer containing 0.5% BSA, 5 mM EDTA with 7-AAD, before being measured by Cytek Aurora Flow Cytometer (Cytek Biosciences, Amsterdam, The Netherlands) and analysed on FlowJo (version 10.9.0 for macOS). ..

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer.
    Article Snippet: .. Cells were stained with 7-AAD for cell death and analyzed on a flow cytometer (Cytek Aurora Spectral Analyzer). .. OT-I CD8+ cells were activated from this culture with 5 μg/ml ovalbumin peptide (OVA257-264, InvivoGen, vac-sin) and expanded for 3 days with or without oxidized lipids (OxPL) and arachidonic acid (AA).

    Labeling:

    Article Title: Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration
    Article Snippet: Briefly, cells were harvested by trypsinization, washed once with annexin V binding buffer (BD Biosciences, USA), and incubated with Annexin V-FITC (BD Biosciences, USA) for 20 min at 4 oC. .. After one wash, cells were labeled with 7-AAD for 15 min at 4oC and immediately analyzed using a Cytek Aurora spectral flow cytometer, with SpectroFlo ® software (Cytek Bioscience, USA). .. In parallel, viability was also assessed using the Cell Death Detection ELISA PLUS kit (Roche, Switzerland), which quantifies cytoplasmic histone-associated DNA fragments as a hallmark of apoptosis.

    Software:

    Article Title: Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration
    Article Snippet: Briefly, cells were harvested by trypsinization, washed once with annexin V binding buffer (BD Biosciences, USA), and incubated with Annexin V-FITC (BD Biosciences, USA) for 20 min at 4 oC. .. After one wash, cells were labeled with 7-AAD for 15 min at 4oC and immediately analyzed using a Cytek Aurora spectral flow cytometer, with SpectroFlo ® software (Cytek Bioscience, USA). .. In parallel, viability was also assessed using the Cell Death Detection ELISA PLUS kit (Roche, Switzerland), which quantifies cytoplasmic histone-associated DNA fragments as a hallmark of apoptosis.

    Staining:

    Article Title: Cryptococcus neoformans responds to presence of Mycobacterium by diversifying its morphologies and remodelling its capsular material
    Article Snippet: Then, cells were blocked by Fc-blocking buffer (anti-CD16/32, BD Biosciences, Wokingham, UK) for 10 min at 4 °C. .. Cells were subsequently stained with anti-CD11b (BUV395, BD Biosciences), anti-CD11c (BV711, BD Biosciences), anti-Siglec-F (CD170 Super Bright 436, Fisher Scientific), anti-CD45 (PerCP-Cy 5.5, BD Biosciences) and anti-F4/80 (565410, BD Biosciences) at 4 °C for 1 h. Cells were then washed with flow cytometry cell buffer containing 0.5% BSA, 5 mM EDTA with 7-AAD, before being measured by Cytek Aurora Flow Cytometer (Cytek Biosciences, Amsterdam, The Netherlands) and analysed on FlowJo (version 10.9.0 for macOS). ..

    Article Title: Tumor cell death by ferroptosis contributes to an immunosuppressive tumor microenvironment in syngeneic murine models of cancer.
    Article Snippet: .. Cells were stained with 7-AAD for cell death and analyzed on a flow cytometer (Cytek Aurora Spectral Analyzer). .. OT-I CD8+ cells were activated from this culture with 5 μg/ml ovalbumin peptide (OVA257-264, InvivoGen, vac-sin) and expanded for 3 days with or without oxidized lipids (OxPL) and arachidonic acid (AA).

    Incubation:

    Article Title: Somatic Miwi2 Modulates Mitochondrial Function in Airway Multiciliated Cells and Exacerbates Influenza Pathogenesis
    Article Snippet: .. After incubation, cells were washedwith PBS, centrifuged at 300xg, and resuspended in FACS buffer supplemented with 7-AAD and analyzed on the Cytek Aurora. e3 iScience 28, 112291, April 18, 2025 qRT-PCR RNA isolation frommouse whole lung homogenate was performed using the Qiagen RNeasymini kit according to themanufacturer’s instructions. qRT-PCR was performed using the TaqMan and QuantStudio 3 (Applied Biosystems 4392938) according to the manufacturer’s instructions. ..

    Quantitative RT-PCR:

    Article Title: Somatic Miwi2 Modulates Mitochondrial Function in Airway Multiciliated Cells and Exacerbates Influenza Pathogenesis
    Article Snippet: .. After incubation, cells were washedwith PBS, centrifuged at 300xg, and resuspended in FACS buffer supplemented with 7-AAD and analyzed on the Cytek Aurora. e3 iScience 28, 112291, April 18, 2025 qRT-PCR RNA isolation frommouse whole lung homogenate was performed using the Qiagen RNeasymini kit according to themanufacturer’s instructions. qRT-PCR was performed using the TaqMan and QuantStudio 3 (Applied Biosystems 4392938) according to the manufacturer’s instructions. ..

    Isolation:

    Article Title: Somatic Miwi2 Modulates Mitochondrial Function in Airway Multiciliated Cells and Exacerbates Influenza Pathogenesis
    Article Snippet: .. After incubation, cells were washedwith PBS, centrifuged at 300xg, and resuspended in FACS buffer supplemented with 7-AAD and analyzed on the Cytek Aurora. e3 iScience 28, 112291, April 18, 2025 qRT-PCR RNA isolation frommouse whole lung homogenate was performed using the Qiagen RNeasymini kit according to themanufacturer’s instructions. qRT-PCR was performed using the TaqMan and QuantStudio 3 (Applied Biosystems 4392938) according to the manufacturer’s instructions. ..



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